Preservation of Integrity of Rat Tissues for Cytochemical Staining Purposes
نویسندگان
چکیده
The use of sucrose solutions as suspending media for preparation of tissue homogenates and subsequent centrifugal fractionation has become a widely accepted procedure. Recently, it has been claimed that addition of colloids to the medium results in better preservation of the morphological and enzymic integrity of the isolated fractions (4, 8, 15). Such media also have valuable applications during the preparation of tissues for eytochemical staining procedures. Brief mention has already been made elsewhere
منابع مشابه
Studies on Formalin Fixation for Electron Microscopy and Cytochemical Staining Purposes
A study has been made of the preservation of fine structure, phospholipids, and the activity of acid phosphatase and esterase in rat liver fixed in various solutions containing 4 per cent formaldehyde. Examination of methacrylate-embedded preparations shows that calcium-containing fixatives result in poor preservation of fine structure, whereas veronal-treated or phosphate-buffered formalin giv...
متن کاملP-74: Cytochemical Evaluation of Sperm Chromatin and DNA Integrity in Couples with 3 Unexplained Recurrent Spontaneous Abortion
Background: The aim of this study was to examine the possible relationship between sperm DNA integrity and chromatin packaging evaluated by cytochemical assays, traditional sperm parameters and recurrent spontaneous abortion (RSA) of unknown origin. Materials and Methods: In this cohort study, 40 couples with a history of RSA and 40 couples with proven fertility were considered as case and cont...
متن کاملP-18: Effects of Ethanol Consumption on Chromatin Condensation and DNA Integrity of Epididymal Spermatozoa In Rat
Background: Alcohol abuse is considered as one of the problems associated with poor semen production and sperm quality. Both acute and chronic alcohol consumption may affect spermatozoal chromatin disorders through apoptosis. Therefore, for the first time, this experimental study was performed to evaluate the effect of ethanol consumption on sperm parameters and chromatin integrity of spermatoz...
متن کاملFormalin Fixation in the Cytochemical Demonstration of Succinic Dehydrogenase of Mitochondria
A variety of established methods for protecting mitochondria were tested on rat duodenal epithelium during the histochemical assay for succinic dehydrogenase. The use of sucrose at isotonic or hypertonic concentrations, 7.5 per cent polyvinylpyrrolidone, divalent cations, physiological salt solutions, phenazine methosulfate, coenzyme Q(10), and menadione failed to improve the quality of the his...
متن کاملComparative Assessment of Nuclear and Nucleolar Cytochemical Parameters of Oral Epithelial Cells in Smokers and Non-Smokers by Methyl Green-Pyronin Staining
Introduction: A strong relationship exists between cigarette smoking and the development of oral squamous cell carcinoma. Smoking can significantly increase cellular proliferation. Nevertheless, there is little reference in literature to the cytological assessment of oral mucosa in this respect. Methods: Changes in nuclear and neucleolar cytomorphometric parameters such as diameter, surface, nu...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- The Journal of Biophysical and Biochemical Cytology
دوره 7 شماره
صفحات -
تاریخ انتشار 1960